Generator

Part:BBa_K3187043:Design

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-13)


Superfolder GFP + P22 Bacteriophage Scaffolding Protein Generator (tetR/tetA promotor)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 1334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal NheI site found at 1339
    Illegal PstI site found at 1334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal BamHI site found at 796
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 1334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 1334
    Illegal NgoMIV site found at 1185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 94


Design Notes

N/A


Source

Ordered from Integrated DNA Technologies (IDT) and cloned in pTetW3con2

References